6H4G

Regulatory subunit of a cAMP-independent protein kinase A from Trypanosoma brucei: E311A, T318R, V319A mutant bound to cAMP in the A site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	64.674, 71.197, 121.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.206 - 2.144
R-factor	0.234430659958
Rwork	0.230
R-free	0.29817
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6flo
RMSD bond length	0.008
RMSD bond angle	0.972
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXCD
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.210	2.240
High resolution limit [Å]	2.144	2.144
Rpim	0.089	0.122
Number of reflections	31343
<I/σ(I)>	0.92
Completeness [%]	100.0
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	50 mM Tris pH 8.0 0.2 mM Magnesium Chloride 40% PEG 3350