6H4G
Regulatory subunit of a cAMP-independent protein kinase A from Trypanosoma brucei: E311A, T318R, V319A mutant bound to cAMP in the A site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-19 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.674, 71.197, 121.397 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.206 - 2.144 |
R-factor | 0.234430659958 |
Rwork | 0.230 |
R-free | 0.29817 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6flo |
RMSD bond length | 0.008 |
RMSD bond angle | 0.972 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXCD |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.210 | 2.240 |
High resolution limit [Å] | 2.144 | 2.144 |
Rpim | 0.089 | 0.122 |
Number of reflections | 31343 | |
<I/σ(I)> | 0.92 | |
Completeness [%] | 100.0 | |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 50 mM Tris pH 8.0 0.2 mM Magnesium Chloride 40% PEG 3350 |