6H3U
Schmallenberg Virus Glycoprotein Gc Head Domain in Complex with scFv 4B6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980097 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 175.842, 175.842, 153.684 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.554 - 3.168 |
| R-factor | 0.2019 |
| Rwork | 0.200 |
| R-free | 0.22840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6H3T along with a Phyre2-generated model of the scFv 4B6 antibody fragment |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.759 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.554 | 3.610 |
| High resolution limit [Å] | 3.168 | 3.170 |
| Rmerge | 0.220 | 1.780 |
| Rmeas | 0.230 | 1.820 |
| Rpim | 0.050 | 0.410 |
| Number of reflections | 27774 | 1391 |
| <I/σ(I)> | 11.4 | 2 |
| Completeness [%] | 59.1 | 9.3 |
| Redundancy | 20.9 | 18.7 |
| CC(1/2) | 1.000 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.75 uL of a 22.8 mg/mL solution of the GPC-purified complex in 20 mM Tris-Cl pH 8.0, 150 mM NaCl were added to 0.75 uL of reservoir solution containing 0.1 M sodium acetate pH 4.6, 3.5 M sodium formate |
