6H2U
Crystal structure of human METTL5-TRMT112 complex, the 18S rRNA m6A1832 methyltransferase at 1.6A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.887, 70.634, 84.776 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.317 - 1.600 |
| R-factor | 0.1872 |
| Rwork | 0.186 |
| R-free | 0.21480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.1) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.240 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.068 | 0.887 |
| Rmeas | 0.075 | 0.978 |
| Rpim | 0.030 | 0.406 |
| Total number of observations | 279014 | |
| Number of reflections | 45777 | 2230 |
| <I/σ(I)> | 14 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.1 | 5.8 |
| CC(1/2) | 0.998 | 0.623 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 25% PEG 4,000; 0.2 M ammonium sulfate; 100 mM Na citrate pH 5.6 |
