6H2U

Crystal structure of human METTL5-TRMT112 complex, the 18S rRNA m6A1832 methyltransferase at 1.6A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	56.887, 70.634, 84.776
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.317 - 1.600
R-factor	0.1872
Rwork	0.186
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless (0.5.1)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.240	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.068	0.887
Rmeas	0.075	0.978
Rpim	0.030	0.406
Total number of observations	279014
Number of reflections	45777	2230
<I/σ(I)>	14
Completeness [%]	99.9	99.9
Redundancy	6.1	5.8
CC(1/2)	0.998	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	25% PEG 4,000; 0.2 M ammonium sulfate; 100 mM Na citrate pH 5.6