6GYT

Transcription factor dimerization activates the p300 acetyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	49.640, 83.710, 165.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.087 - 2.500
R-factor	0.2619
Rwork	0.261
R-free	0.28820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bhw
RMSD bond length	0.012
RMSD bond angle	1.578
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.600	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	23996
<I/σ(I)>	7.6
Completeness [%]	97.3
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		294	1.6 M Ammonium Sulfate, 100 mM Bicine, pH 9.0