6GY5

Crystal structure of the kelch domain of human KLHL20 in complex with DAPK1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	297
Detector technology	PIXEL
Collection date	2017-09-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	40.459, 47.361, 151.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.650 - 1.086
R-factor	0.1543
Rwork	0.153
R-free	0.17240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vpj
RMSD bond length	0.010
RMSD bond angle	1.333
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.650	29.650	1.090
High resolution limit [Å]	1.060	4.740	1.060
Rmerge	0.071	0.066	0.448
Rmeas	0.085	0.077	0.620
Rpim	0.045	0.040	0.427
Number of reflections	130575	1699	7488
<I/σ(I)>	11.8	26.1	1.4
Completeness [%]	97.6	99.5	77.3
Redundancy	5.6	5.9	2.2
CC(1/2)	0.997	0.995	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	2M Sodium Chloride, 0.1M Acetate pH 4.5