6GXC

Bacterial oligosaccharyltransferase PglB in complex with an inhibitory peptide and a reactive lipid-linked oligosaccharide analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	83.810, 116.540, 173.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.094 - 3.401
R-factor	0.2522
Rwork	0.249
R-free	0.28310
RMSD bond length	0.008
RMSD bond angle	1.157
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (v1.12)
Refinement software	PHENIX (v1.12)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.094	3.490
High resolution limit [Å]	3.401	3.401
Rmeas	0.069	1.440
Number of reflections	24002
<I/σ(I)>	23.28
Completeness [%]	92.1	33.7
Redundancy	13.4	4.8
CC(1/2)	0.999	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.4	293	0.1M glycine, pH 9.4, 0.15M magnesium acetate, and 30% PEG400