6GXC
Bacterial oligosaccharyltransferase PglB in complex with an inhibitory peptide and a reactive lipid-linked oligosaccharide analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.810, 116.540, 173.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.094 - 3.401 |
| R-factor | 0.2522 |
| Rwork | 0.249 |
| R-free | 0.28310 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.157 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (v1.12) |
| Refinement software | PHENIX (v1.12) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.094 | 3.490 |
| High resolution limit [Å] | 3.401 | 3.401 |
| Rmeas | 0.069 | 1.440 |
| Number of reflections | 24002 | |
| <I/σ(I)> | 23.28 | |
| Completeness [%] | 92.1 | 33.7 |
| Redundancy | 13.4 | 4.8 |
| CC(1/2) | 0.999 | 0.676 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.4 | 293 | 0.1M glycine, pH 9.4, 0.15M magnesium acetate, and 30% PEG400 |
