6GWS

Crystal structure of human PCNA in complex with three p15 peptides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-30
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	79.240, 89.750, 85.130
Unit cell angles	90.00, 117.25, 90.00

Refinement procedure

Resolution	75.690 - 2.900
R-factor	0.203
Rwork	0.200
R-free	0.26320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vym
RMSD bond length	0.020
RMSD bond angle	2.396
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	89.750	8.560	3.080
High resolution limit [Å]	2.900	6.110	2.900
Rmerge	0.105	0.064
Rmeas		0.090
Number of reflections	42698	2948	6844
<I/σ(I)>	4.77	10.04	0.73
Completeness [%]	92.2	93.4	91.3
Redundancy	1.768	1.846	1.762
CC(1/2)	0.986	0.986	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	28% PEG 400, 0.2 M CaCl2, 0.1 M Hepes