6GVY
Mutant M16A of RNA dependent RNA polymerase 3D from Foot-and-Mouth disease Virus complexed with an template -primer RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.073, 94.073, 99.806 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.590 - 2.200 |
| R-factor | 0.23484 |
| Rwork | 0.233 |
| R-free | 0.27425 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wne |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.907 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.052 | |
| Rpim | 0.027 | |
| Number of reflections | 26375 | |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% PEG 4000, 0.2M magnesium acetate, 0.1M MES[2-(N-morpholino) ethanesulfonic acid] pH 6.0 and 4% butyrolactone. |
