6GRO
Human CSNK1G3 bound to SB-223133
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-03 |
| Detector | DECTRIS PILATUS3 R CdTe 300K |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.750, 56.750, 221.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.980 - 1.450 |
| R-factor | 0.13503 |
| Rwork | 0.134 |
| R-free | 0.16732 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2izr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.413 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.150 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.140 | |
| Number of reflections | 74333 | 3637 |
| <I/σ(I)> | 6.2 | 2.2 |
| Completeness [%] | 99.6 | 98 |
| Redundancy | 4.4 | 4.2 |
| CC(1/2) | 0.975 | 0.617 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2 M sodium/potassium tartrate, 0.1 M bis-tris-propane pH 8.5, 20% PEG3350, 10% ethylene glycol |
