6GQF

The structure of mouse AsterA (GramD1a) with 25-hydroxy cholesterol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	52.435, 121.012, 71.907
Unit cell angles	90.00, 110.84, 90.00

Refinement procedure

Resolution	67.203 - 2.900
R-factor	0.2039
Rwork	0.201
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yqj
RMSD bond length	0.004
RMSD bond angle	0.782
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.203	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.100	0.627
Rmeas	0.105	0.662
Rpim	0.033	0.210
Number of reflections	18439	2958
<I/σ(I)>	13.6	3.7
Completeness [%]	99.0	98.7
Redundancy	10	9.8
CC(1/2)	0.998	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.2 M NaCl, 0.1 M sodium cacodylate pH6, 8% PEG 8000