6GQF
The structure of mouse AsterA (GramD1a) with 25-hydroxy cholesterol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.435, 121.012, 71.907 |
| Unit cell angles | 90.00, 110.84, 90.00 |
Refinement procedure
| Resolution | 67.203 - 2.900 |
| R-factor | 0.2039 |
| Rwork | 0.201 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yqj |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.782 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.203 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.100 | 0.627 |
| Rmeas | 0.105 | 0.662 |
| Rpim | 0.033 | 0.210 |
| Number of reflections | 18439 | 2958 |
| <I/σ(I)> | 13.6 | 3.7 |
| Completeness [%] | 99.0 | 98.7 |
| Redundancy | 10 | 9.8 |
| CC(1/2) | 0.998 | 0.891 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.2 M NaCl, 0.1 M sodium cacodylate pH6, 8% PEG 8000 |
