6GPG

Structure of the RIG-I Singleton-Merten syndrome variant C268F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	175.620, 175.620, 109.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.468 - 2.894
R-factor	0.2121
Rwork	0.210
R-free	0.25970
RMSD bond length	0.009
RMSD bond angle	1.238
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.468	3.000
High resolution limit [Å]	2.894	2.894
Rmerge	0.076
Number of reflections	22649
<I/σ(I)>	19.72
Completeness [%]	99.7
Redundancy	13.09

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M MOPS pH 7.5, 17.5 % (w/v) PEG 3350, 0.25 M NaSCN, 3 % (v/v) 2,2,2 trifluoroethanol