6GOA
Structural basis for OXA-48 dimerization - R189A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184109 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.726, 85.545, 125.805 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 24.670 - 2.550 |
| R-factor | 0.2406 |
| Rwork | 0.239 |
| R-free | 0.27480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5qb0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.571 |
| Data reduction software | XDS (20180126) |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHASER (2.8) |
| Refinement software | PHENIX (dev_3150) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.670 | 2.641 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmeas | 0.206 | |
| Number of reflections | 72154 | |
| <I/σ(I)> | 5 | 1.09 |
| Completeness [%] | 96.2 | 96.56 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.982 | 0.568 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M BIS-Tris-propane pH 9.5, 16% PEG MME 5000 |
