6GMQ

pVHL:EloB:EloC in complex with (4-(1H-pyrrol-1-yl)phenyl)methanol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 41 2 2
Unit cell lengths	93.830, 93.830, 363.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.672 - 2.755
R-factor	0.2092
Rwork	0.205
R-free	0.27940
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.920
High resolution limit [Å]	2.750	2.750
Number of reflections	43083
<I/σ(I)>	21.4	5.2
Completeness [%]	99.2
Redundancy	13.1
CC(1/2)	0.999	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	0.1 M Na cacodylate pH 6.0, 0.2 M Mg acetate, 15% PEG3350, 5mM DTT