6GKT

X-ray structure of a non-autocrystallizing galectin-10 variant, Gal10-Tyr69Glu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99998
Spacegroup name	P 1 21 1
Unit cell lengths	72.823, 93.296, 93.068
Unit cell angles	90.00, 108.94, 90.00

Refinement procedure

Resolution	42.000 - 2.100
R-factor	0.174
Rwork	0.172
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lcl
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.100	2.100
Rmeas	0.150	2.270
Number of reflections	68207	10924
<I/σ(I)>	9.33	0.7
Completeness [%]	99.0	98.6
Redundancy	6.9	6.7
CC(1/2)	0.990	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M ammonium citrate pH 5.1 20% PEG 3350