6GKF

Structure of 14-3-3 gamma in complex with caspase-2 14-3-3 binding motif Ser139

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-07
Detector	RAYONIX MX225HE
Wavelength(s)	0.9184
Spacegroup name	P 41 21 2
Unit cell lengths	122.540, 122.540, 312.013
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.183 - 2.598
R-factor	0.2428
Rwork	0.241
R-free	0.28880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b05
RMSD bond length	0.002
RMSD bond angle	0.367
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.183	2.691
High resolution limit [Å]	2.598	2.598
Rmerge	0.129	1.658
Rmeas	0.132	1.689
Rpim	0.025	0.320
Number of reflections	74062	7245
<I/σ(I)>	23.63	2.17
Completeness [%]	99.8	99.59
Redundancy	26.7	27.5
CC(1/2)	1.000	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293.15	PEG 4000, TRIS-HCl, sodium acetate