6GK7
Crystal structure of anti-tau antibody dmCBTAU-27.1, double mutant (S31Y, T100I) of CBTAU-27.1, in complex with Tau peptide A8119B (residues 299-318)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.829, 60.524, 91.048 |
| Unit cell angles | 90.00, 104.37, 90.00 |
Refinement procedure
| Resolution | 88.200 - 2.950 |
| R-factor | 0.22352 |
| Rwork | 0.220 |
| R-free | 0.26778 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.383 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.200 | 3.200 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.128 | 0.457 |
| Number of reflections | 10377 | |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 98.0 | 98.9 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG8000 |
