6GJB
Erk2 signalling protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-20 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54718 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.804, 70.596, 60.023 |
| Unit cell angles | 90.00, 109.20, 90.00 |
Refinement procedure
| Resolution | 24.320 - 1.820 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.20600 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.460 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmeas | 0.045 | 0.884 |
| Number of reflections | 34405 | 2609 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 98.7 | 93.7 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 0.2M ammonium sulphate, 34% MPEG2000, 0.1MHepes, pH7.3, 0.02M mercaptoethanol |
