6GG1

Structure of PROSS-edited human interleukin 24

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	38.920, 65.369, 67.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.960 - 1.300
R-factor	0.1676
Rwork	0.161
R-free	0.18345
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n1f
RMSD bond length	0.010
RMSD bond angle	1.525
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.960	46.960	1.320
High resolution limit [Å]	1.300	7.120	1.300
Rmerge	0.069	0.075
Rmeas	0.074	0.081
Rpim	0.028	0.029
Number of reflections	43156	327	2091
<I/σ(I)>	14.7	42.9	1.3
Completeness [%]	100.0	99.9	99.9
Redundancy	13	11.8	11.9
CC(1/2)	0.999	0.997	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	20% PEG8000, 0.2M CaCl2, 0.1M MES