6GFE
High-resolution Structure of a therapeutic full-length anti-NPRA Antibody with exceptional Conformational Diversity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.780, 160.510, 87.300 |
| Unit cell angles | 90.00, 110.72, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.800 |
| R-factor | 0.1819 |
| Rwork | 0.181 |
| R-free | 0.20020 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS ((VERSION January 19) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.967 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.045 | 0.548 |
| Rmeas | 0.054 | |
| Number of reflections | 155361 | 15552 |
| <I/σ(I)> | 16.64 | 2.28 |
| Completeness [%] | 99.5 | 99.81 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 5 % PEG 8000, 0.2 M Calcium acetate, 0.1 M imidazole |
