6GE5

TEAD4 (216-434);Y429F COMPLEXED WITH YAP PEPTIDE (60-100) AND MYRISTOATE (COVALENTLY BOUND) AT 2.05A (P41212 CRYSTAL FORM); MYRISTOYLATION WAS DONE BY ADDING MYR-COA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99994
Spacegroup name	P 41 21 2
Unit cell lengths	58.952, 58.952, 159.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.500 - 2.050
R-factor	0.2234
Rwork	0.223
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ge3
RMSD bond length	0.006
RMSD bond angle	0.964
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.500	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.119	0.863
Rmeas	0.124	0.898
Total number of observations	237255
Number of reflections	18467
<I/σ(I)>	20	3.6
Completeness [%]	99.8	100
Redundancy	12.8	12.8
CC(1/2)	0.999	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	20% PEG 3350, 8% Tacsimate