6GC5
Molecular basis for AU-rich element recognition and dimerization by the HuR C-terminal RRM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-10-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.287, 40.258, 106.496 |
| Unit cell angles | 90.00, 132.95, 90.00 |
Refinement procedure
| Resolution | 77.946 - 1.900 |
| R-factor | 0.2023 |
| Rwork | 0.201 |
| R-free | 0.23120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hi9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.216 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.950 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.046 | 0.668 |
| Rmeas | 0.054 | |
| Number of reflections | 35681 | 3148 |
| <I/σ(I)> | 15.23 | |
| Completeness [%] | 95.1 | 81.19 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.999 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 20 mM Tris (pH8), 100 mM NaCl, 10% (w/v) Glycerol, 1mM TCEP precipitant: 2 M Ammonium sulfate, 0.1 M Bis-Tris well |
