6GBS

Crystal Structure of the C. themophilum Scavenger Decapping Enzyme DcpS apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0004
Spacegroup name	P 1
Unit cell lengths	52.595, 69.719, 70.947
Unit cell angles	104.48, 101.39, 111.61

Refinement procedure

Resolution	48.503 - 1.946
R-factor	0.1845
Rwork	0.183
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bv3
RMSD bond length	0.007
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.503	48.503	2.000
High resolution limit [Å]	1.946	8.720	1.950
Rmerge	0.086	0.038	0.705
Rmeas	0.102	0.045	0.834
Number of reflections	115497	596	4454
<I/σ(I)>	9.82	26.57	2.1
Completeness [%]	96.3	85.8	95.8
Redundancy	3.428	3.351	3.545
CC(1/2)	0.996	0.996	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	200 mM Ammoniumacetate, 100 mM BisTRIS pH 5.5, 25 % PEG 3350