6GB5
Structure of H-2Db with truncated SEV peptide and GL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 91.930, 123.360, 99.206 |
| Unit cell angles | 90.00, 103.73, 90.00 |
Refinement procedure
| Resolution | 75.940 - 2.300 |
| R-factor | 0.2002 |
| Rwork | 0.198 |
| R-free | 0.23976 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7u |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.561 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.940 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.075 | 0.597 |
| Number of reflections | 47383 | 4668 |
| <I/σ(I)> | 7 | 1.9 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 2M ammonium sulfate, 0.1M Tris-HCl (pH 7.5) and 0.5M NaCl |
