6G9B
Crystal structure of Ebolavirus glycoprotein in complex with imipramine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 114.216, 114.216, 305.552 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.458 - 2.260 |
| R-factor | 0.1974 |
| Rwork | 0.196 |
| R-free | 0.22490 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.913 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.000 | 2.300 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.083 | |
| Number of reflections | 36357 | 1787 |
| <I/σ(I)> | 18.5 | 1.2 |
| Completeness [%] | 99.9 | 98.3 |
| Redundancy | 18.5 | 11.4 |
| CC(1/2) | 1.000 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 293 | 9% (w/v) PEG 6000 and 0.1 M Sodium citrate tribasic dihydrate |
