6G6L

The crystal structures of Human MYC:MAX bHLHZip complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979507
Spacegroup name	P 1
Unit cell lengths	48.680, 74.330, 80.060
Unit cell angles	107.12, 107.67, 90.05

Refinement procedure

Resolution	46.155 - 2.200
R-factor	0.2173
Rwork	0.215
R-free	0.26810
RMSD bond length	0.003
RMSD bond angle	0.510
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.540	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.088	0.443
Rmeas	0.109	0.557
Rpim	0.064	0.333
Number of reflections	41701	6326
<I/σ(I)>	6.7	1.9
Completeness [%]	80.7	83.3
Redundancy	2.3	2.3
CC(1/2)	0.995	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	10% w/v PEG 8000, 20% v/v ethylene glycol, 15% PEG 8000 15, 0.2M ammonium sulfate, pH 7