6G63

RNase E in complex with sRNA RrpA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-01
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.987
Spacegroup name	P 61
Unit cell lengths	110.630, 110.630, 466.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.947 - 3.950
R-factor	0.2757
Rwork	0.275
R-free	0.29620
RMSD bond length	0.005
RMSD bond angle	1.012
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.800	4.190
High resolution limit [Å]	3.950	3.950
Rmerge	0.150
Number of reflections	28217
<I/σ(I)>	8.3
Completeness [%]	100.0
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M KCl, 0.01 M MgCl2, 0.05 M Tris-HCl pH 8.5 and 30% (v/v) PEG 400