6G5Y
The X-ray structure of the adduct formed in the reaction between lysozyme and a platinum(II) terpyridine compound (acid pH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-22 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96771 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.490, 78.490, 36.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.500 - 1.490 |
| R-factor | 0.19566 |
| Rwork | 0.193 |
| R-free | 0.24002 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.078 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.510 | 1.570 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.039 | 0.514 |
| Rpim | 0.025 | 0.219 |
| Number of reflections | 18842 | |
| <I/σ(I)> | 18.1 | 3.1 |
| Completeness [%] | 98.3 | 100 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 1.000 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 283 | 20% ethylene glicol, 0.10 M sodium acetate pH 4.4, 0.60 M sodium nitrate |
