6G4T
The crystal structure of uninhibited C183S/C217S mutant of human CA VII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-01-29 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.300, 89.380, 44.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.490 - 1.910 |
| Rwork | 0.196 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ml5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.423 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.500 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.079 | 0.520 |
| Number of reflections | 20846 | |
| <I/σ(I)> | 14.7 | 2.03 |
| Completeness [%] | 98.8 | 87.3 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 25% (w/v) polyethylene glycol 3350, 0.1 M Tris, pH 8.5 |
