6G4Q

Structure of human ADP-forming succinyl-CoA ligase complex SUCLG1-SUCLA2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 31 2 1
Unit cell lengths	130.200, 130.200, 119.772
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.100 - 2.590
R-factor	0.2096
Rwork	0.207
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1euc
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.100	2.660
High resolution limit [Å]	2.590	2.590
Number of reflections	36946	2712
<I/σ(I)>	14.4	2.1
Completeness [%]	100.0	100
Redundancy	9.7
CC(1/2)	0.999	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	2.1M DL- malic acid