6G39
Crystal structure of haspin F605Y mutant in complex with 5-iodotubercidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.820, 78.990, 79.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.010 - 1.450 |
| R-factor | 0.15856 |
| Rwork | 0.158 |
| R-free | 0.17543 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ouc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.644 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.010 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.060 | 0.570 |
| Number of reflections | 86860 | 12366 |
| <I/σ(I)> | 14.2 | 3 |
| Completeness [%] | 98.3 | 96.9 |
| Redundancy | 5.4 | 5.4 |
| CC(1/2) | 0.997 | 0.801 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 51-63% MPD and 0.1M SPG buffer, pH 6.0-6.5 |
