6G39

Crystal structure of haspin F605Y mutant in complex with 5-iodotubercidin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Serine/threonine-protein kinase haspin	polymer	357	40727.5	1	UniProt (Q8TF76) Pfam (PF12330)	Homo sapiens (Human)	Germ cell-specific gene 2 protein,H-haspin,Haploid germ cell-specific nuclear protein kinase
2	B (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
3	C (A)	(2R,3R,4S,5R)-2-(4-AMINO-5-IODO-7H-PYRROLO[2,3-D]PYRIMIDIN-7-YL)-5-(HYDROXYMETHYL)TETRAHYDROFURAN-3,4-DIOL	non-polymer		392.1	1	Chemie (5ID)
4	D (A)	IODIDE ION	non-polymer		126.9	1	Chemie (IOD)
5	E, F (A)	(4R)-2-METHYLPENTANE-2,4-DIOL	non-polymer		118.2	2	Chemie (MRD)
6	G (A)	water	water		18.0	339	Chemie (HOH)

Sequence modifications

A: 465 - 798 (UniProt: Q8TF76)

PDB	External Database	Details
Met 442	-	initiating methionine
His 443	-	expression tag
His 444	-	expression tag
His 445	-	expression tag
His 446	-	expression tag
His 447	-	expression tag
His 448	-	expression tag
Ser 449	-	expression tag
Ser 450	-	expression tag
Gly 451	-	expression tag
Val 452	-	expression tag
Asp 453	-	expression tag
Leu 454	-	expression tag
Gly 455	-	expression tag
Thr 456	-	expression tag
Glu 457	-	expression tag
Asn 458	-	expression tag
Leu 459	-	expression tag
Tyr 460	-	expression tag
Phe 461	-	expression tag
Gln 462	-	expression tag
Ser 463	-	expression tag
Met 464	-	expression tag
Tyr 605	Phe 605	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	40727.5
Non-Polymers*	Number of molecules	5
	Total formula weight	778.4
All*	Total formula weight	41505.9

*Water molecules are not included.