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6G27

X-ray structure of NSD3-PWWP1 in complex with compound 5

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06SA
Synchrotron siteSLS
BeamlineX06SA
Temperature [K]100
Detector technologyPIXEL
Collection date2015-03-07
DetectorDECTRIS PILATUS 6M-F
Wavelength(s)1.000
Spacegroup nameP 21 21 21
Unit cell lengths44.466, 48.116, 63.494
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution38.350 - 1.650
R-factor0.207
Rwork0.206
R-free0.22700
RMSD bond length0.008
RMSD bond angle0.870
Data reduction softwareXDS
Data scaling softwareAimless (0.3.11)
Phasing softwareBALBES
Refinement softwareBUSTER (2.11.7)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]38.3501.653
High resolution limit [Å]1.6481.648
Rmerge0.0610.800
Rmeas0.0670.440
Rpim0.0260.170
Number of reflections170315840
<I/σ(I)>15.42.2
Completeness [%]100.0100
Redundancy6.56.6
CC(1/2)0.9980.960
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION277100mM Morpheus Buffer 3, 30% P550MME_P20K, 10% Morpheus Ethylene glycols

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