6G1E

BEAT Fc with improved heterodimerization (Q3A-D84.4Q)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2015-11-20
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966
Spacegroup name	P 21 21 21
Unit cell lengths	49.650, 73.020, 140.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.880
R-factor	0.23329
Rwork	0.231
R-free	0.27247
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m3v
RMSD bond length	0.007
RMSD bond angle	1.372
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.980
High resolution limit [Å]	1.880	1.880
Rmerge	0.080	0.622
Rmeas	0.090	0.707
Rpim	0.041	0.332
Number of reflections	40305	2929
<I/σ(I)>	8.5
Completeness [%]	99.5
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	CRYSTALS GROWN BY VAPOR DIFFUSION BY MIXING 12.0 MG/ML PROTEIN IN 10 MM HEPES PH8.0, 100 MM NACL, 1 MM EDTA, EQUALLY WITH 33.00 %(W/V) PEG 1500