6G0A

The crystal structure of the Pol2 catalytic domain of DNA polymerase epsilon carrying a P301R substitution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9840
Spacegroup name	C 1 2 1
Unit cell lengths	159.895, 67.252, 151.639
Unit cell angles	90.00, 111.50, 90.00

Refinement procedure

Resolution	19.977 - 2.620
R-factor	0.226
Rwork	0.224
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m8o
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	78.780	78.780	2.710
High resolution limit [Å]	2.620	10.150	2.620
Rmerge	0.128	0.086	0.859
Rmeas	0.156	0.110	1.054
Rpim	0.087	0.068	0.601
Total number of observations	136067	2411	12302
Number of reflections	44773	830	4351
<I/σ(I)>	5.9	18.1	1.3
Completeness [%]	98.7	97.8	98.2
Redundancy	3	2.9	2.8
CC(1/2)	0.829	0.653	0.368

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	50mM MES pH 6.5, 150mM NaAc and 8% PEG20K