6FZT
Crystal structure of Smad8_9-MH1 bound to the GGCGC site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-04 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.967 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.433, 79.509, 88.372 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.110 - 2.460 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fzs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.110 | 2.630 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rpim | 0.040 | 0.550 |
| Number of reflections | 15793 | 791 |
| <I/σ(I)> | 14.3 | 1.3 |
| Completeness [%] | 92.0 | 54.3 |
| Redundancy | 6 | 6.3 |
| CC(1/2) | 0.998 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M NaF, 0.1 M bis-tris propane pH 8.5, 20% PEG3350 |
