6FZL
Crystal structure of human transthyretin double mutant K35T/T119M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 43.056, 63.580, 85.957 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.979 - 1.446 |
| R-factor | 0.1819 |
| Rwork | 0.181 |
| R-free | 0.19150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f41 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.691 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER (1.7.3-928) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 63.580 | 63.580 | 1.451 |
| High resolution limit [Å] | 1.446 | 6.711 | 1.446 |
| Rmerge | 0.046 | 0.023 | 0.849 |
| Rmeas | 0.050 | 0.025 | 0.927 |
| Rpim | 0.020 | 0.011 | 0.369 |
| Total number of observations | 267716 | ||
| Number of reflections | 41465 | 512 | 402 |
| <I/σ(I)> | 19.7 | ||
| Completeness [%] | 96.6 | 100 | 96.6 |
| Redundancy | 6.5 | 5.7 | 6.2 |
| CC(1/2) | 0.999 | 0.999 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 7% glycerol, 1.3 M sodium citrate, pH 5.5 |
