6FYW

Structure of B/Brisbane/60/2008 Influenza Hemagglutinin in complex with SD83

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-15
Detector	MAR CCD 130 mm
Wavelength(s)	1.0331
Spacegroup name	I 21 3
Unit cell lengths	189.040, 189.040, 189.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.260 - 2.200
R-factor	0.1933
Rwork	0.193
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fqm
RMSD bond length	0.003
RMSD bond angle	0.612
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2614: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rpim	0.060	0.440
Number of reflections	56754	5669
<I/σ(I)>	9.9
Completeness [%]	100.0	100
Redundancy	5.1	5.1
CC(1/2)	1.000	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	0.1 M MES, pH 6.5 and 10% PEG 20,000