6FYK
X-Ray structure of CLK2-KD(136-496)/Indazole1 at 2.39A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99986 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 95.633, 95.633, 222.858 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.680 - 2.390 |
| R-factor | 0.1964 |
| Rwork | 0.195 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fyi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.680 | 2.450 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.163 | 0.927 |
| Rmeas | 0.171 | 0.981 |
| Number of reflections | 47518 | |
| <I/σ(I)> | 14.4 | 2.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10 | 9.5 |
| CC(1/2) | 0.998 | 0.859 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 20% PEG2000, 0.2M trimethylamine n-oxide, 0.1M TRIS |
