6FY5
Crystal structure of the macro domain of human macroh2a2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97565 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.750, 41.370, 84.150 |
| Unit cell angles | 90.00, 97.05, 90.00 |
Refinement procedure
| Resolution | 19.680 - 1.650 |
| R-factor | 0.216 |
| Rwork | 0.215 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zr3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.690 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmeas | 0.060 | 1.100 |
| Number of reflections | 46778 | 2724 |
| <I/σ(I)> | 13.15 | 1.25 |
| Completeness [%] | 97.3 | 92.6 |
| Redundancy | 5.4 | 3.4 |
| CC(1/2) | 0.999 | 0.450 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 12.5% (w/v) PEG 1,000, 0.2 M sodium acetate, 0.1 M Mes pH 6.5 |
