6FQP

Crystal structure of TALE homeobox domain transcription factor TGIF1 with its consensus DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.972422
Spacegroup name	P 21 21 21
Unit cell lengths	50.851, 67.248, 102.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.537 - 2.420
R-factor	0.2069
Rwork	0.205
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xrm
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.250	2.510
High resolution limit [Å]	2.420	2.420
Rmerge	0.028	0.232
Number of reflections	13814
<I/σ(I)>	17.3
Completeness [%]	98.9	98.7
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M sodium acetate trihydrate pH 4.5, 30% v/v PEG 1500