6FQH

GluA2(flop) S729C ligand binding core dimer bound to NBQX at 1.76 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 61
Unit cell lengths	108.107, 108.107, 99.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.053 - 1.759
R-factor	0.191023582719
Rwork	0.189
R-free	0.22398
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kgc
RMSD bond length	0.011
RMSD bond angle	1.172
Data reduction software	xia2
Data scaling software	Aimless (0.5.26)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.053	1.790
High resolution limit [Å]	1.759	1.760
Rmeas	0.041
Rpim	0.016
Number of reflections	65151
<I/σ(I)>	23.1
Completeness [%]	100.0
Redundancy	6.7
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	0.1M tri-sodium Citrate, 20% PEG 3000