6FNW

Structure of a volume-regulated anion channel of the LRRC8 family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-11-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1, 2.05
Spacegroup name	P 65
Unit cell lengths	140.130, 140.130, 42.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.452 - 1.800
R-factor	0.1805
Rwork	0.179
R-free	0.20820
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.270
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.452	1.900
High resolution limit [Å]	1.800	1.800
Rmeas		1.580
Number of reflections	44375	6557
<I/σ(I)>	27.05	2.27
Completeness [%]	100.0	99.9
Redundancy	20.2	19.8
CC(1/2)		0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277.15	22% PEG 3350, 0.2M Na/K tartrate, 0.1M Bis-Tris propane pH 8.5