6FNL

Crystal Structure of Ephrin B4 (EphB4) Receptor Protein Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918400
Spacegroup name	P 1 21 1
Unit cell lengths	46.138, 54.061, 62.119
Unit cell angles	90.00, 110.87, 90.00

Refinement procedure

Resolution	43.112 - 1.269
R-factor	0.1968
Rwork	0.197
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vwu
RMSD bond length	0.007
RMSD bond angle	0.834
Data reduction software	XDS (XDSapp 2)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.112	1.340
High resolution limit [Å]	1.269	1.269
Rmeas	0.069
Number of reflections	74570	11399
<I/σ(I)>	12.14	0.23
Completeness [%]	97.8	93.1
Redundancy	6.69	6.4
CC(1/2)	0.999	0.126

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	25 % PEG5000 MME, 15 % Glycerol, 0.1 M TRIS pH7.5