6FLN

Crystal structure of the human TRIM25 coiled-coil and PRYSPRY domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.072
Spacegroup name	P 61 2 2
Unit cell lengths	89.920, 89.920, 827.090
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	137.850 - 3.600
R-factor	0.28877
Rwork	0.287
R-free	0.31844
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Separate coiled-coil (PDB:4LTB) and PRYSPRY (PDB:6FLM) domains
RMSD bond length	0.008
RMSD bond angle	1.051
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	137.850	3.690
High resolution limit [Å]	3.600	3.600
Rmerge	0.136	2.380
Rmeas	0.151	2.640
Number of reflections	24552
<I/σ(I)>	8.18
Completeness [%]	99.5	98.5
Redundancy	5.36	5.51
CC(1/2)	0.999	0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein at 10 mg/ml was mixed in 2:1 ratio with mother liquor containing 0.2 M sodium formate, 14% PEG 3350 and 0.1 M bis-Tris pH 6.5.