6FLM

Crystal structure of the human TRIM25 PRYSPRY domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-14
Detector	MARRESEARCH
Wavelength(s)	0.8726
Spacegroup name	P 3 2 1
Unit cell lengths	152.750, 152.750, 68.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.735 - 2.009
R-factor	0.167
Rwork	0.165
R-free	0.20610
Structure solution method	SIRAS
RMSD bond length	0.005
RMSD bond angle	0.707
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.735	2.100
High resolution limit [Å]	2.009	2.010
Rmerge	0.124	0.336
Rmeas	0.135	0.412
Number of reflections	52640
<I/σ(I)>	11.42	2.36
Completeness [%]	84.1
Redundancy	5.34
CC(1/2)	0.996	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	15 mg/ml protein mixed in 1:1 ratio with reservoir containing 0.1 M bis-TRIS pH 6.5 and 2M ammonium sulphate.