6FIB
Structure of human 4-1BB ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.99993 |
| Spacegroup name | P 62 |
| Unit cell lengths | 119.592, 119.592, 104.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 103.570 - 2.700 |
| R-factor | 0.169 |
| Rwork | 0.166 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x29 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.600 | 3.042 |
| High resolution limit [Å] | 2.697 | 2.697 |
| Number of reflections | 14507 | 725 |
| <I/σ(I)> | 21.6 | |
| Completeness [%] | 91.4 | 64.4 |
| Redundancy | 11.4 | 11 |
| CC(1/2) | 1.000 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris, pH 8.5, 20% PEG1000, 0.009M BaCl2 |
