6FHZ

Inward-facing conformation of a multidrug resistance MATE family transporter of the MOP superfamily.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-05
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	107.120, 134.310, 69.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.803 - 2.800
R-factor	0.2306
Rwork	0.229
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mlb
RMSD bond length	0.001
RMSD bond angle	0.360
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.700	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.223
Rmeas	0.238
Rpim	0.082	0.749
Number of reflections	12731	1263
<I/σ(I)>	8.8	0.9
Completeness [%]	99.8	99.8
Redundancy	8.3	5.5
CC(1/2)	0.996	0.296

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5	295	30% PEG 600, 100 mM Na3C6H5O7-C6H8O7, 100 mM NaCl, 100 mM MgCl2