6FGO
Fc in complex with engineered calcium binding domain Z
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 90.229, 120.296, 126.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.529 - 2.500 |
R-factor | 0.2048 |
Rwork | 0.203 |
R-free | 0.23190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5u4y |
RMSD bond length | 0.003 |
RMSD bond angle | 0.575 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER |
Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.530 | 46.529 | 2.640 |
High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
Rmerge | 0.031 | 0.420 | |
Rmeas | 0.067 | 0.035 | 0.474 |
Rpim | 0.030 | 0.016 | 0.213 |
Number of reflections | 47055 | 1542 | 6882 |
<I/σ(I)> | 13.8 | 18.4 | 1.8 |
Completeness [%] | 98.0 | 93.9 | 99.3 |
Redundancy | 4.4 | 4.2 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 24 % PEG3350, 0.1 M LiCl2, 0.1 M MES pH 6.0 |