6FGA
Crystal structure of TRIM21 E3 ligase, RING domain in complex with its cognate E2 conjugating enzyme UBE2E1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.278383 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.835, 95.871, 235.097 |
| Unit cell angles | 90.00, 93.15, 90.00 |
Refinement procedure
| Resolution | 47.900 - 2.820 |
| R-factor | 0.25492 |
| Rwork | 0.253 |
| R-free | 0.29407 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.467 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.912 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.076 | 1.050 |
| Rmeas | 0.082 | 1.130 |
| Rpim | 0.031 | 0.420 |
| Number of reflections | 51064 | 2694 |
| <I/σ(I)> | 17 | 1.8 |
| Completeness [%] | 91.2 | 50.9 |
| Redundancy | 6.81 | 7.2 |
| CC(1/2) | 0.999 | 0.705 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100mM Bicine pH 9.0 and 5% w/v PEG 6000 |
