6FG2
CRYSTAL STRUCTURE OF FAB OF NATALIZUMAB IN COMPLEX WITH FAB OF NAA84.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-25 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.404, 66.619, 199.502 |
| Unit cell angles | 90.00, 98.97, 90.00 |
Refinement procedure
| Resolution | 55.210 - 2.788 |
| R-factor | 0.239 |
| Rwork | 0.238 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4irz 3S37 and 4OD2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 197.060 | 3.143 |
| High resolution limit [Å] | 2.788 | 2.788 |
| Rmerge | 0.143 | |
| Number of reflections | 40020 | |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | MES 100mM - PEG 400 35% - NaCl 200mM - MPD 4% - pH6 |
